Immunoprecipitation and immunoblotting analysis of VCP interaction partners in <i>H. pylori</i>-infected AGS cells.

<p>AGS Cells were transfected with plasmid coding for VCP-Flag or empty vector, pretreated with MG-132 for 3 hr, and incubated for 6 hr with <i>H. pylori</i>. Immunoprecipitations were performed with anti-Flag M2 antibody affinity gel and the immunoprecipitates analyzed by immunoblotting to identify VCP interaction partners. 1% of input was subjected to immunoblotting.