Challenge of ookinete cultures with increasing concentrations of the ROS producing agent paraquat (PQ).

<p>RT-qPCR data showing dose-response modulation of target gene expression in ookinete cultures in the presence of increasing concentrations of the superoxide-producing compound Paraquat (PQ, Viologen, Aldrich). Increasing concentrations of PQ were added to 12-hours <i>P. berghei</i> ookinete cultures as indicated. Shown are the ratios of normalized relative transcript quantities (RQ) of treated vs. non-treated samples (non-treated RQ = 1, represented by the dashed line). All data were normalized against the expression of 18 s rRNA A-type <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003136#ppat.1003136-Yano1" target="_blank">[29]</a>, <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003136#ppat.1003136-Thompson1" target="_blank">[34]</a>. (*) indicates statistical significance (p<0.1). Shown are mean values of 3 independent experiments. Error bars indicate STDEV.