Isobaric tags for relative and absolute quantitation (iTRAQ) is a prominent mass spectrometry technology for protein identification and quantification that is capable of analyzing multiple samples in a single experiment. Frequently, iTRAQ experiments are carried out using an aliquot from a pool of all samples, or “masterpool”, in one of the channels as a reference sample standard to estimate protein relative abundances in the biological samples and to combine abundance estimates from multiple experiments. In this manuscript, we show that using a masterpool is counterproductive. We obtain more precise estimates of protein relative abundance by using the available biological data instead of the masterpool and do not need to occupy a channel t...
The accuracy of quantification obtained using the iTRAQ labelling methodology for measuring protein ...
Background: iTRAQ(TM) technology for protein quantitation using mass spectrometry is a recent, power...
iTRAQ labeling of peptides is widely used for quantitative comparison of biological samples using ma...
iTRAQ (isobaric tags for relative or absolute quantitation) is a mass spectrometry technology that a...
iTRAQ (isobaric tags for relative or absolute quantitation) is a mass spectrometry technology that a...
There is considerable interest in using mass spectrometry for biomarker discovery in human blood pla...
iTRAQ labeling of peptides is widely used for quantitative comparison of biological samples using ma...
Quantitative comparison of the protein content of biological samples is a fundamental tool of resear...
The recent introduction of isobaric peptide tags for relative and absolute quantification (iTRAQ) of...
Quantitative proteomic analysis can help elucidating unexplored biological questions; it, however, r...
Large-scale proteomic analysis is emerging as a powerful technique in biology and relies heavily on ...
Shotgun proteomic methods involving isobaric tagging of peptides enable high-throughput proteomic an...
This dataset/code forms part of Andrew Landels' thesis: "Improving proteomic methods and investigati...
Reporter ion-based methods are among the major techniques to quantify peptides and proteins. Two mai...
This study aimed to compare the depth and reproducibility of total proteome and differentially expre...
The accuracy of quantification obtained using the iTRAQ labelling methodology for measuring protein ...
Background: iTRAQ(TM) technology for protein quantitation using mass spectrometry is a recent, power...
iTRAQ labeling of peptides is widely used for quantitative comparison of biological samples using ma...
iTRAQ (isobaric tags for relative or absolute quantitation) is a mass spectrometry technology that a...
iTRAQ (isobaric tags for relative or absolute quantitation) is a mass spectrometry technology that a...
There is considerable interest in using mass spectrometry for biomarker discovery in human blood pla...
iTRAQ labeling of peptides is widely used for quantitative comparison of biological samples using ma...
Quantitative comparison of the protein content of biological samples is a fundamental tool of resear...
The recent introduction of isobaric peptide tags for relative and absolute quantification (iTRAQ) of...
Quantitative proteomic analysis can help elucidating unexplored biological questions; it, however, r...
Large-scale proteomic analysis is emerging as a powerful technique in biology and relies heavily on ...
Shotgun proteomic methods involving isobaric tagging of peptides enable high-throughput proteomic an...
This dataset/code forms part of Andrew Landels' thesis: "Improving proteomic methods and investigati...
Reporter ion-based methods are among the major techniques to quantify peptides and proteins. Two mai...
This study aimed to compare the depth and reproducibility of total proteome and differentially expre...
The accuracy of quantification obtained using the iTRAQ labelling methodology for measuring protein ...
Background: iTRAQ(TM) technology for protein quantitation using mass spectrometry is a recent, power...
iTRAQ labeling of peptides is widely used for quantitative comparison of biological samples using ma...