Phospho-flow analysis of human IL-4 DC stimulated by LPS.

<p>Human IL-4 DC were activated by different LPS for 2 min, 5 min, 10 min, 30 min, 60 min and 180 min. A phospho-flow analysis using fluorescent cell barcoading was performed in order to assess the phosphorylation levels of molecules involved in TLR4 signaling. The heatmap visualization of phosphorylation changes is shown. The median fluorescent intensity (MFI) of stimulated cells is normalized by MFI of medium stimulated cells. Colored bar on the right shows the levels of fold changes. Experiments were performed on 4 different donors. The data for one representative are shown.