Addition of microglia to neurons reduces mEPSC frequency.

<p>Primary hippocampal neurons (DIV21) were cultured for 2 days either alone or in the presence of microglia obtained from MacGreen mice. <b>A.</b> Neurons were stained with anti-NeuN antibody and visualized with Alexa Fluor555-conjugated secondary antibody. Scale bar, 50 µm. <b>B.</b> mEPSC recordings from neurons in the presence or absence of microglia. <b>C, D.</b> Summary of mean mEPSC frequency (<b>C</b>) and amplitude (<b>D</b>) from neurons in the presence or absence of microglia.