<p>Fibrils prepared from recombinant α-syn were incubated with increasing concentrations of [<sup>125</sup>I]SIL23. Nonspecific binding was determined in parallel reactions utilizing 50 µM ThioT as competitor. A representative plot of specific binding versus [<sup>125</sup>I]SIL23 concentration is shown in <b>A</b>. Data points represent mean +/− s.d. (n = 3). The data was analyzed by curve fitting to a one-site binding model using nonlinear regression. The K<sub>d</sub> value was determined by fitting the data to the equation Y = B<sub>max</sub>*X/(X+K<sub>d</sub>). Scatchard analysis of binding is shown in <b>B</b>. Similar results were obtained in more than three independent experiments.</p
Measuring the rate and extent of radioligand binding provides information on the number of binding s...
<p>Association rate constants (<i>k</i><sub>a</sub>) were plotted against dissociation rate constant...
[125I]-NKA binding to human NK2 receptors at increasing radioligand concentrations. Saturation data ...
<p>Binding affinities of [<sup>125</sup>I]SIL23 to Aβ (<b>A</b>) and tau (<b>B</b>) fibrils were det...
<p>K<sub>i</sub> values were calculated from EC<sub>50</sub> values using the equation K<sub>i</sub>...
<p>α-Syn fibrils were incubated with 200 nM [<sup>125</sup>I]SIL23 and increasing concentrations of ...
<p>Homogenized insoluble fractions from human brain samples (n = 8) were incubated with increasing c...
<div><p>Accumulation of α-synuclein (α-syn) fibrils in Lewy bodies and Lewy neurites is the patholog...
<p>Recruitment of <sup>125</sup>I-labeled AL and MM κ (A) or λ (B) LC proteins by rVλ6Wil fibrils (e...
<p>Levels of α-syn were measured in insoluble fractions from human brain samples (n = 10) by sequent...
Accumulation of a-synuclein (a-syn) fibrils in Lewy bodies and Lewy neurites is the pathological hal...
<p>(A) Specific binding of [<sup>125</sup>I]-rIFI16 on the plasma membrane of HUVEC, HeLa, HACAT, HD...
The reversible combination of a ligand with specific sites on the surface of a receptor is one of th...
<p>Purified collagen I-monomers were coated to microtiter plates and incubated with <sup>125</sup>I-...
<p>The peptide was injected in solution at three different concentrations, for 5 mins at a flow rate...
Measuring the rate and extent of radioligand binding provides information on the number of binding s...
<p>Association rate constants (<i>k</i><sub>a</sub>) were plotted against dissociation rate constant...
[125I]-NKA binding to human NK2 receptors at increasing radioligand concentrations. Saturation data ...
<p>Binding affinities of [<sup>125</sup>I]SIL23 to Aβ (<b>A</b>) and tau (<b>B</b>) fibrils were det...
<p>K<sub>i</sub> values were calculated from EC<sub>50</sub> values using the equation K<sub>i</sub>...
<p>α-Syn fibrils were incubated with 200 nM [<sup>125</sup>I]SIL23 and increasing concentrations of ...
<p>Homogenized insoluble fractions from human brain samples (n = 8) were incubated with increasing c...
<div><p>Accumulation of α-synuclein (α-syn) fibrils in Lewy bodies and Lewy neurites is the patholog...
<p>Recruitment of <sup>125</sup>I-labeled AL and MM κ (A) or λ (B) LC proteins by rVλ6Wil fibrils (e...
<p>Levels of α-syn were measured in insoluble fractions from human brain samples (n = 10) by sequent...
Accumulation of a-synuclein (a-syn) fibrils in Lewy bodies and Lewy neurites is the pathological hal...
<p>(A) Specific binding of [<sup>125</sup>I]-rIFI16 on the plasma membrane of HUVEC, HeLa, HACAT, HD...
The reversible combination of a ligand with specific sites on the surface of a receptor is one of th...
<p>Purified collagen I-monomers were coated to microtiter plates and incubated with <sup>125</sup>I-...
<p>The peptide was injected in solution at three different concentrations, for 5 mins at a flow rate...
Measuring the rate and extent of radioligand binding provides information on the number of binding s...
<p>Association rate constants (<i>k</i><sub>a</sub>) were plotted against dissociation rate constant...
[125I]-NKA binding to human NK2 receptors at increasing radioligand concentrations. Saturation data ...