Binding of [¹²⁵I]SIL23 is correlated with levels of insoluble α-syn present in PD brain.

<p>Levels of α-syn were measured in insoluble fractions from human brain samples (n = 10) by sequential extraction and western blot. A representative syn1 western blot with SDS extracts from PD and control cases is shown in <b>A</b>. A quantitative syn303 western blot with SDS extracts from PD cases is shown in <b>B</b>, in which higher molecular weight α-syn species (asterisk) are observed in combination with monomeric α-syn (arrow). The correlation of B_max values for [¹²⁵I]SIL23 binding to levels of total insoluble α-syn quantified from the monomer band on western blot is shown in <b>C</b>. The correlation of B_max values for [¹²⁵I]SIL23 binding to levels of total insoluble α-syn quantified from monomer plus high molecular weight species on western blot is shown in <b>D</b>. Results were verified with more than three independent experiments.