Immunofluorescence-LM of proteins in intact trichomes.

<p>Cultures grown in MLA₀ and a 12L/12D cycle were sampled 3 h after the beginning of the light phase. Immunofluorescence was performed on <i>C. raciborskii</i> using antibodies against (A) NtcA, (B) FtsZ, (C) RbcL proteins and against the NifH protein in both (D) <i>C. raciborskii</i> and (E) <i>Anabaena</i> PCC7120. (F) <i>A. variabilis</i> ATCC29413 grown in BG11₀ and a 12L/12D cycle was used as a positive control for NifH (<i>nif</i>2 nitrogenase) presence in vegetative cells (when sampled 4 h in the dark phase). Arrows indicate the positions of heterocysts.