Chloroplast responses to BL in <i>Arabidopsis</i> leaves pretreated with neomycin. A and B

<p>Parameters of chloroplast responses in WT plants and <i>phot1</i> mutant after application of 10 mM PIPES, 10, 100 or 250 µM neomycin: (A) amplitudes and (B) kinetics. Data values are mean ± SE (n = 10) from 3 independent experiments. The minus values on the graphs represent the parameters for weak BL response (1.6 µmol m⁻²s⁻¹) and the plus values indicate the strong BL response parameters (120 µmol m⁻²s⁻¹). <b>C, D, E and F</b> The chloroplast positioning in the mesophyll cell layer. Dark-acclimated <i>Arabidopsis</i> WT leaf tissues were irradiated with strong BL (100 µmol m⁻²s⁻¹ for 60 min) followed by weak BL (2 µmol m⁻²s⁻¹ for 60 min) in the (C) absence or (D, E, F) presence of neomycin (10, 100, 250 µM). Images from time point 0 min, 60 min and 120 min are shown, taken at 40X. Arrow show chloroplasts at anticlinal cell wall and arrowhead show chloroplasts at periclinal cell wall. Bar = 20 µm.