Inhibition of the proteasome with the drug MG-132 induced formation of PPAT-EGFP bodies in a time-dependent manner.

<p>HeLa cells treated with 20 µM MG-132 in DMSO for 1.5 hours, 2.5 hours, 5 hours, and 8 hours showed increasing fractions of the cell population exhibiting PPAT-EGFP puncta. Control treatments with only the carrier (DMSO) for 8 hours showed only minimal penetrance, consistent with no treatment (<b>Fig. 1B</b>). Bars indicate average +/- 1 s. d. across 3 replicates, <i>n</i> = 625, 793, 1024, 466, and 397 cells, respectively.