CpG methylation differentiates normal epithelial cell types from adenoma.

<p>a) Schematic representation of intestinal tissues and cell types. Differentiated villus cells are the prevailing component of bulk normal tissue samples. b) Colour-coded table of CpG methylation analyses of 11 DMRs, using bisulfite pyrosequencing. <i>Cd133</i> is an intestinal stem cell and cancer stem cell marker. <i>Dusp6</i> to <i>Slc25a28</i> represent adenoma hypomethylated DMRs, <i>Ush1g</i>_1 to <i>Vdr</i> represent adenoma hypermethylated DMRs. Percent CpG methylation within the regions are given as numbers and colour-code. Dark blue, <20% CpG methylation; light blue, 20–50% CpG methylation, light red, 50–80% CpG methylation; bright red, >80% methylation. c) Hierarchical clustering of methylation data (as shown in b) separates adenoma from normal tissue and cell preparations. Pearson correlation was employed.