CncC and dKeap1 subcellular localization and polytene chromosome occupancy.

<p>(A). Conserved sequences and mutations in CncC, CncB and dKeap1. Regions that are conserved between <i>Drosophila</i> and mammalian proteins are shown in color. The locations of the <i>cnc^K6</i> and <i>dKeap1^EY5</i> mutations are indicated <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003263#pgen.1003263-Sykiotis1" target="_blank">[3]</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003263#pgen.1003263-Veraksa1" target="_blank">[15]</a>. (B). Visualization of the subcellular distributions of CncC and dKeap1 in salivary glands. [Upper panels] Endogenous CncC and dKeap1 distributions in wild type (<i>w¹¹¹⁸</i>) salivary glands stained using anti-CncC or anti-dKeap1 (green) and anti-Lamin Dm0 (red) antibodies. [Middle panels] Wild type (<i>w¹¹¹⁸</i>) salivary glands were stained with anti-CncC or anti-dKeap1 antibodies (yellow) and Hoechst (blue). <i>[Lower panels]</i> Ectopic CncC and dKeap1 distributions in live salivary glands that expressed rxYFP-CncC and rxYFP-dKeap1, respectively. The intrinsic fluorescence (yellow) was superimposed on Hoechst fluorescence (blue). The salivary glands were isolated from early wandering 3^rd instar larvae. The scale bars are 10 µm. (C). Visualization of the localization of endogenous dKeap1 in the prothoracic gland and in imaginal disc cells. Endogenous dKeap1 was visualized by immunostaining using anti-dKeap1 antibodies (yellow) superimposed on Hoechst staining (blue). The separate images are shown to the right of each color image. (D). Visualization of the loci occupied by CncC and dKeap1 on polytene chromosomes. [Upper panels] The loci occupied by endogenous CncC and dKeap1 were visualized on polytene chromosome from wild type larvae (<i>w¹¹¹⁸</i>) by staining with anti-CncC and anti-dKeap1 antibodies, respectively. [Lower panels] The loci occupied by ectopic CncC and dKeap1 were visualized on polytene chromosomes from larvae that expressed rxYFP-CncC and rxYFP-dKeap1, respectively, by staining with anti-GFP antibodies. The immunofluorescence (yellow) was superimposed on Hoechst fluorescence (blue). The major ecdysone-regulated early puffs are indicated. The polytene chromosomes were prepared from the salivary glands of early wandering 3^rd instar larvae.