Efflux of ethidium bromide by <i>A. baylyi</i> ADP1 cells.

<p>Wild-type and mutant strains were loaded with 15 µM ethidium bromide in the presence of the protonophore CCCP. Cells were energised using 0.4% glucose at the point indicated by an arrow to initiate efflux. Efflux was monitored fluorometrically using an excitation wavelength of 530 nm and emission wavelength of 610 nm. Assays were conducted in duplicate, and a representative experiment is shown.