WNV replication in permissive and nonpermissive tissues is controlled by RLR and type I IFN signaling.

<p>Viral burden analysis of spleen (permissive) and liver (nonpermissive) tissues from WT, <i>Mavs^−/−</i>, <i>Ifnar^−/−</i>, and <i>Mavs^−/−</i>×<i>Ifnar^−/−</i> (DKO) mice infected subcutaneously in the footpad with 100 PFU of WNV-TX. Viral burden in the (<b>A</b>) spleen and (<b>B</b>) liver were determined by plaque assay. Data are represented as PFU per gram (n = 4 mice per timepoint). (<b>C</b>) Viral burden in WT infected livers determined by qRT-PCR using virus-specific primers and represented as a relative ratio of WNV to GAPDH RNA (n = 3–4) mice per timepoint). Graphs show the mean +/− standard deviation for each measurement. Asterisk denotes <i>P</i><0.05. The horizontal line indicates the lower limit of assay sensitivity. BLD = below limit of detection. n.a. = not applicable.