The roof plate formation in the <i>mib</i> mutant.

<p>(A), Confocal images, lateral view of the spinal cord of <i>mib</i> mutant, 72 hpf. Dashed rectangular shows the magnified in (A′) region of the spinal cord with the absence of GFP-positive RP cells. (B, C), the orthogonal optical sections of confocal images of the spinal cord of <i>mib</i> mutant illustrate lack of the roof plate extension between 36 and 72 hpf. Immunofluorescent staining of the transverse sections of spinal cord using anti-GFP (green) and anti-GFAP (red) antibodies; wild-type embryo (D–F) and <i>mib</i> mutant (G–I).