Comparison between the endo-metabolome of BY4741 parental strain and of <i>fps1Δ</i> deletion mutant.

<p>Yeast cells were harvested during the exponential phase of growth (OD_{600 nm} = 1.0) in the same basal medium supplemented with 0 or 2% v/v of ethanol. (<b>A</b>) 2D score plot displaying the space formed by the two first principal components (R²X for PC1 equal to 0.525 and R²X for PC2 equal to 0.321), (<b>B</b>) 2D loading plot displaying the space formed by the two first principal components (with characteristic bins for a set of metabolites) and (<b>C</b>) relative abundances of different metabolites in the endo-metabolome of the parental strain and the <i>fps1Δ</i> deletion strain during the exponential growth phase (OD_{600 nm} = 1.0) in the presence of different ethanol concentrations (0 and 2% v/v). Experimental values are means of six independent experiments with standard deviation error bars. Key: <i>1 </i>NAD⁺, <i>2 </i>NADP⁺, <i>3</i> L-tyrosine, <i>4</i> Fumaric acid, <i>5</i> L-phenylalanine, <i>6</i> Citric acid, <i>7</i> Succininic acid, <i>8</i> Glycerol, <i>9</i> L-glutamic acid, <i>10</i> L-leucine, <i>11</i> L-methionine, 12 L-alanine, <i>13 </i>L-ornithine, <i>14</i> L-threonine, <i>15</i> L-valine,<i>16</i> L-glutamine, <i>17 </i>L-aspartic acid, <i>18</i> L-malic acid, <i>19</i> L-histidine, <i>20</i> L-serine, <i>21</i> L-asparagine, <i>22</i> L-lysine, <i>23</i> Thiamine, <i>24</i> NADH and <i>25</i> L-proline.