Generation of ApoA1 transgenic mice.

<p>Overexpression of hApoA1 was induced using doxycycline-containing drinking water (Doxy+). Control mice were offered normal water (Doxy−). (<i>A)</i> RT-PCR analysis revealed hApoA1 mRNA only in the lungs of mice treated with doxycycline. (<i>B)</i> ApoA1 protein expression was significantly greater in the lungs of doxycycline-treated transgenic mice compared with those of wild-type or transgenic mice not treated with doxycycline (<i>n</i> = 6 mice/group). **<i>p<</i>0.01 compared with WT or Doxy(−) mice. (C) Detection of human ApoA1 in BAL fluids. ELISA was performed on the first 1-mL fraction of BAL fluid, with a detection limit of 3.13 ng/mL (dashed line). Values below the detection limit were treated as 0 (<i>n</i> = 6 mice/group). **<i>p<</i>0.01 compared with Doxy(−) mice. (D<i>)</i> Immunofluorescence analysis showed that ApoA1 was strongly expressed in the alveolar epithelium (white arrow heads) in the doxycycline-treated transgenic mice. Scale bar = 10 µm. H&E, hematoxylin and eosin.