Gene composition of transgenic lines.

<p>Tail DNA for the transgenic lines indicated above the lanes were amplified for the gene segment indicated to the left of each set of PCR results. After amplification in the presence of ³²P-dATP, the products were digested with a restriction enzyme. Restriction fragments that arise from the transgene only (Tg) or the endogenous genes only (End) are indicated. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055842#pone.0055842.s003" target="_blank">Table S3</a> for PCR primers, restriction digests, and predicted fragment sizes. The designation of these fragments are consistent with the DNA sequences of the relevant C57BL/6 or strain 129 DNA, and have been confirmed by comparison to C57BL/6 DNA <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055842#pone.0055842-Dunnick1" target="_blank">[18]</a>. Other restriction fragments are shared by both the transgene and endogenous genes.