Characterization of CD90/Thy-1-immunoreactive cells with lymphocyte morphology.

<p>A–C) Double-labeling of a precision cut lung slices with antibodies against CD90/Thy-1 (green) and CD45 (red), and subsequent quantitative analysis (D) shows that more than 96% of CD90/Thy-1-immunoreactive cells with lymphocyte morphology are also stained with CD45 (white arrowheads, cells positive for CD90/Thy-1and CD45; red arrow, cell immunoreactive for CD90/Thy-1 only). In each animal, 90 to 150 CD90/Thy-1-immunoreactive cells were analyzed. E–G). Double-labeling with antibodies against CD90/Thy-1 (green) and CD3 (red) indicates that most CD90/Thy-1-labeled cells with lymphocyte morphology are also CD3-immunoreactive.