Blockade of CD40/CD40L signaling in short- versus long-term DC/T-cell Co-cultures.

<p>(A) CD40L expression on CD4⁺ T-cells activated after 2, 4 and 6 hours of CD3/CD28 cross-linking is shown. One representative out of 4 experiments is depicted. (B) Short- or long-term co-culture of CD3⁺ T-cells and DCs, activated with LPS/IFN-γ for 6 hours, in the presence of blocking CD40 and CD40L monoclonal antibodies (black bars) or isotype control immunoglobulins (white bars) is shown. Median±SEM of 7 to 12 experiments is depicted. DCs from Donors A, B and D were used in experiments (A) and (B).