Sexual crosses of the WT and Δbcpls1 mutant.

<p><b>A:</b> Sexual crosses between <i>B. cinerea</i> SAS405 microconidia (standard strain of the opposite mating type to B05.10) and sclerotia of the B05.10 WT, two independent Δbcpls1 transformants and the Δbcpls1:GFP-Pls1 complementation strain. Sclerotia of the Δbcpls1 deletion strain show no morphological differences during their development but are sterile. <b>B:</b> Sexual crosses between <i>B. cinerea</i> SAS405 sclerotia and microconidia of the B05.10 WT, the Δbcpls1 mutants and the Δbcpls1:GFP-Pls1 complementation strains. Microconidia of the Δbcpls1 deletion strain are fertile.