<i>ssb<sub>X</sub></i> is expressed in <i>hrp</i>-inducing conditions and regulated by HrpG and HrpX.

  • Yu-Rong Li (5651359)
  • Wen-Xiu Ma (281803)
  • Yi-Zhou Che (281804)
  • Li-Fang Zou (5651362)
  • Muhammad Zakria (281805)
  • Hua-Song Zou (5651365)
  • Gong-You Chen (143244)
Publication date
February 2013

Abstract

<p>(A) Schematic map of a transcriptional fusion where the <i>ssb<sub>Xoc</sub></i> promoter of <i>X. oryzae</i> pv. <i>oryzicola</i> RS105 is fused to the <i>gusA</i> reporter gene. Upper panel shows pPIPAGUS containing the <i>ssb<sub>Xoc</sub></i> promoter and an imperfect PIP-box (TTCGC-N<sub>19</sub>-TTCGT) fused with a promoter-less <i>gusA</i> gene. Lower panel shows pPIPBGUS with a mutated <i>ssb<sub>Xoc</sub></i> promoter (the first TT nucleotides replaced with AA) fused with <i>gusA</i>. (B) â-glucuronidase (GUS) activity in the <i>hrp</i>-inducing medium XOM3. Plasmids pPIPAGUS and pPIPBGUS were transferred into the wild-type RS105 and mutants RΔ<i>hrpG</i> and RΔ<i>hrpX</i>. The recombinant strains were then grown in <i>hrp</i>-i...

Extracted data

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