Mycobacteria-induced expression of MCP-2/CCL8 through TLR2.

<p>(A-B) Real-time PCR of <i>mcp-2/ccl8</i> mRNA in primary peritoneal macrophages from wildtype, TLR2^−/−, TLR3^−/−, or TLR4^−/− mice infected with either <i>M. bovis</i> BCG (A) or <i>M. tuberculosis</i> H37Rv (B) at MOI 5 for 24 h. (B) Western blot of phosphorylation of Akt and p38 in primary peritoneal macrophages from wildtype or TLR2 KO mice infected with <i>M. bovis</i> BCG at MOI 5 for indicated times. Data shown are representative of three independent experiments. (D) Densitometric analysis of three independent experiments as in (C). Data shown are the mean±sem of three independent experiments. **, <i>p</i><0.001, *, <i>p</i><0.05.