(a) Typical stepping trajectory, i.e., spatial displacement as a function of time and (b) dwell time distribution of myosin V, adapted from [4].

<p>(a) In single-molecule experiments with a feedback loop, the data are monitored under constant external load. Hence, the distance between the bead monitoring the motor’s motion (upper gray trajectory, with the thin black line showing a filtered curve) and the trap center (black trajectory) remains constant. (b) Dwell time distribution of myosin V for saturating [ATP]. The solid line is a fit from Ref. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055366#pone.0055366-Rief1" target="_blank">[4]</a> that involves two exponential functions with decay rates 150/s and 12.5/s.