Sequence and Southern blot analyses of mtDNA from mtDNA mutator mice.

<p>Total DNA was prepared from the hearts of a 10-month-old wild-type mouse (WT) and an age matched homozygous mutator mouse (m/m), and from the trans-mitochondrial cybrids carrying mtDNA from platelets of a 10-month-old wild-type mouse and age-matched m/m mutator mouse, respectively. (A) Sequence analysis of 517 bp-fragments of the <i>COX1</i> gene. Numbers of somatic mutations in mtDNA of the hearts from a wild-type mouse and an m/m mouse, and of the cybrids with mtDNA of a wild-type and an m/m mouse were counted. (B) Southern blot analysis of <i>Xho</i>I-digested mtDNA. Lanes 1 and 2 represent the hearts of a WT and an m/m mouse, respectively. Lanes 3 and 4 represent the cybrids with mtDNA of a wild-type and an m/m mouse, respectively.