LPS analysis of <i>S.</i> Enteritidis strains overexpressing RcsB (A) or PmrA (B) protein.

<p>Equal amount of LPS was loaded in each lane and analyzed by Tricine/SDS-PAGE on a 14% (w/v) acrylamide gel followed by silver staining. The concentration of LPS was determined by measuring KDO using the purpald assay. <b>A.</b> Lanes 1–4: bacteria grown in N-minimal medium containing 10 mM MgCl₂; lanes 5–8: bacteria grown in N-minimal medium containing 10 µM MgCl₂ 100 µM FeSO₄. <b>B.</b> Lanes 1–4: bacteria grown in N-minimal medium containing 10 mM MgCl₂; lanes 5–7: bacteria grown in N-minimal medium containing 10 µM MgCl₂ 100 µM FeSO₄. Plasmids pIZ833, p<i>rcsB</i> and p<i>pmrA</i> bears the <i>dam</i>, <i>rcsB</i> and <i>pmrA</i> genes respectively.