miR-199a-5p expression during bleomycin induced lung fibrosis.

<p>(A) Heat map representing the statistically significant (adjusted p-value<0.05) differentially expressed microRNAs in lungs from BALB/C and C57BL/6 mice in response to bleomycin at the indicated time points. Up-regulated microRNAs are shown in progressively brighter shades of red, depending on the fold difference, and down-regulated microRNAs are shown in progressively brighter shades of green. miR-199a-5p is marked in red. n = 3 mice in each group. (B) miR-199a-5p expression in lungs from BALB/C and C57BL/6 mice in response to bleomycin at the indicated time points. n = 3 mice in each group. Data from microarrays experiments are expressed as mean of normalized fluorescence intensity ± SEM. **p<0.01 (C) Paraffin sections were prepared from lungs of C57BL/6 mice 14 days following bleomycin intra-tracheal instillation. <i>In situ</i> hybridization and immunohistochemistry assays were performed to determine the colocalization of miR-199a-5p and α-SMA. Results represent one out of three independently performed experiments.