IgG anti-PvMSP-3α in mice immunized with various adjuvant formulations.

<p>Groups of 6 female BALB/c mice were immunized 3 times (s.c.) with 10 µg of protein in the following adjuvant formulations: Alum, FliC, CpG ODN 1826, Quil A, TiterMax, or IFA. Anti-PvMSP-3α in the sera of immunized mice was analyzed by ELISA 2 weeks after the first (<b>A</b>), second (<b>B</b>), and third (<b>C</b>) immunizing dose. Results are expressed as mean IgG antibody titers (log₁₀) ± SEM and were compared by one-way ANOVA followed by Tukey’s test for multiple comparisons. Significant differences are noted on the graph: *<i>p</i><0.05; **<i>p</i><0.01; ***<i>p</i><0.001. Non-significant (n.s.) differences are indicated (<i>p</i>>0.05). Data representative of 2 independent experiments.