eNOS function in flow-conditioned endothelial cells.

<p>(A): eNOS mRNA expression (presented as mean±S.E.M.) was upregulated under either perfusion condition, but no statistical difference between flow groups was observed. (B): After 0, 12, or 24 hours of conditioning, media was collected and samples analyzed using a fluorometric assay. Total NO byproduct accumulation was normalized by the mean cell count at the end of each period. Results are displayed as mean±SEM (n = 4–6). Asterisks denote significant differences in individual means between groups at each time point. Dagger (†) denotes a significant difference in mean with respect to t = 0 hours. Double dagger (‡) denotes significance with respect to t = 0 and t = 12 hours.