Alignment, structure, and experimental data for p38 MAP kinase (CMGC_sub1).

<p>(<b>A</b>). 1KV2 with <b>15</b> bound. Residues that differ between mammals and nematodes within 5 Å of the active site are colored in grey, and residues within 10 Å are shown in blue. The compound is colored yellow. (<b>B</b>). Dose-response curve of <b>15</b> in <i>C. elegans</i>. (<b>C</b>). Expression data from <i>B. malayi</i> microarray data <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003149#ppat.1003149-Li3" target="_blank">[66]</a> for p38 orthologs in <i>B. malayi</i>. (<b>D</b>). Percent reduction in microfilarial release in <i>B. malayi</i> cultured with <b>15</b> <i>in vitro</i>. (<b>E</b>). Percent reduction in viability in <i>B. malayi</i> female and male worms cultured with <b>15</b>. (<b>F</b>). 1KV2 alignment of Tsp_3128 (<i>T. spiralis</i>), Mh10g200708_Contig883_17801_19594 (<i>M. hapla</i>), 14956.m00538 (<i>B. malayi</i>), B0218.3 (<i>C. elegans</i>), CBN12041 (<i>C. brenneri</i>), CBG01555 (<i>C. briggsae</i>), NP001095644.1 (<i>B. taurus</i>), ENSP00000229795 (<i>H. sapiens</i>), ENSMUSP00000004990 (<i>M. musculus</i>), P70618.3 (<i>R. norvegicus</i>), NP_001003206.1 (<i>C. familaris</i>), 1KV2. Residues 5 and 10 Å from the compound in the active site are starred. Differences in the active site are represented by larger red star at the bottom.