Gene expression in <i>Sclerotinia sclerotiorum</i> Inv- and Inv+ isolates.

<p>6A) Positions of RT-PCR primers on <i>S. sclerotiorum</i> Inv- (top) and Inv+ (bottom) <i>MAT</i> loci, the <i>MAT</i> inversion region is indicated by a gray horizontal line. Genes are colored boxes, white and dotted boxes within genes correspond to alpha1 and HMG domains, respectively, flanking genes are white boxes, directions of transcription are indicated by arrows, gene names are inside or by the boxes. Dashed box and arrow represent <i>MAT1-1-1</i> 3’-fragment lacking an in frame start codon. Diagrams are to scale. The Inv+ alpha1 box is truncated after 45 bp and is not illustrated, for details see text. 6B) Gels with RT-PCR results for all <i>MAT</i> genes, the <i>MAT1-1-1</i> 3’-fragment and an actin control, for all eight <i>S. sclerotiorum</i> strains 1B331-1 – 1B331-8 representing an ordered tetrad. Gene names are indicated above the gels. Lane numbers refer to <i>S. sclerotiorum</i> strain identifiers 1B331-1– 1B331-8. The letters M and N indicate size markers and negative controls, respectively. Band sizes are indicated on the left of each gel. For <i>MAT1-1-1</i> and <i>MAT1-2-1,</i> the sizes of the transcript variants are indicated on the right of the gels.