<i>C. trachomatis</i> transformants expressing RSGFP, mCherry or CFP from the <b><i>incD</i></b><b> gene promoter</b>.

<p>A–C Maps of the p2TK-SW2 derivatives allowing expression of RSGFP (A), mCherry (B) or CFP (C) from the <i>incDEFG</i> operon promoter. The pSW2 plasmid is shown in black, the <i>E.coli</i> origin of replication in dark grey and the ampicillin resistance cassette (<i>bla</i>) in light grey. The <i>rsgfp</i> (green), <i>mCherry</i> (red) and <i>cfp</i> (blue) ORFs are flanked by the <i>incDEFG</i> operon promoter and terminator (light grey). Unique restriction sites are shown in red. D–F. Immunofluorescence images of <i>C. trachomatis</i> inclusions harboring bacteria expressing RSGFP (D), mCherry (E) or CFP (F) from the <i>incDEFG</i> operon promoter. Scale Bar: 10 µm.