The effect of iNOS inhibition on HSV-sr39tk/GCV and IL-3 therapies.

<p>(A) Gene expression of CD11b⁺ cells isolated from tumors as analyzed by RT-PCR. When tumor diameters reached 6–8 mm, tumors were resected and digested to produce a single cell suspension. CD11b⁺ cells were isolated using a MACS CD11b MicroBeads cell separation kit. Total RNA was extracted, reverse transcribed, and amplified with specific primers for 30 cycles. (B) Representative images of iNOS expression from mice in each group. iNOS gene expression was analyzed by immunohistochemical staining. Tumor tissue was stained using anti-iNOS (green) and anti-CD11b/CD68 (red) antibodies. White arrows indicate iNOS⁺/CD11b⁺ or CD68⁺ cells and white stars indicate iNOS⁺/CD11b⁻ or CD68⁻ cells. Original magnification: ×400. Scale bar: 50 µm. Images are representative results from individual animals in each group. (C) Percentages of CD11b⁺iNOS⁺ and CD11b⁻iNOS⁺ were quantified by Image-Pro Plus 6.0 software. *indicates <i>P</i><0.05 determined by the Student’s t-test.