Normal effector and memory CD8+ T cell response to Listeria in KSR1-deficient mice.

<p>WT and KSR1-/- mice were infected with 2×10³ cfu of Lm-OVA, and presence of OVA epitope specific CD8+ T cells in the spleen was analyzed by flow cytometry 7 (A, B) and 30 (C, D, G, H) days after infection. 30 days after Lm-OVA infection, mice were challenged with 10⁵ cfu of Lm-OVA and presence of OVA epitope specific CD8+ T cells in the spleen was assessed 5 days later (E, F). A, C, E: Percentage of Db-GP33 Tetramer positive cells in CD8+ T cells. Plots are representative of two experiments with 3–8 mice per group. B, D, F: Number of Db-GP33 Tetramer+ CD8+ T cells per spleen. Dashed line represents average numbers in naive mice and mice infected with Lm. G, H: CD62L and CD127 expression in Db-GP33 Tetramer+ CD8+ T splenocytes 30 days after Lm-OVA infection. Data is presented as mean + SEM and is representative of two experiments with 3–8 mice per group. There were no significant differences between WT and KO mice (t-test).