Knockdown of ANT2 enhances Apo2L/TRAIL-induced apoptosis by upregulating DR5 at the post-transcriptional level.

<p>DU145 cells were transfected with two different ANT2 siRNAs (siANT2 #1 and #2) or a non-targeting siRNA (siCtrl) and were incubated for 48 hr. A, ANT2 mRNA was quantified by real-time RT-PCR and normalized by GAPDH. B, The cells were treated with or without 50 ng/ml Apo2L/TRAIL. After 24 hr, the sub-G1 population of the cells was measured with flow cytometry. C, Protein levels of DR5 and β-actin were analyzed by Western blotting. D, mRNA levels of DR5 were measured by real-time RT-PCR and normalized by GAPDH. Columns, mean; bars, SD (n = 3). *<i>P</i><0.05, **<i>P</i><0.01 relative to control.