Morbillivirus V proteins show varied abilities to co-precipitate STAT1 and STAT2.

<p>Vero human-SLAM cells were transfected in duplicate wells with 1 µg of empty vector or plasmid driving the expression of the indicated protein. Forty-eight hours post-transfection, one member of each pair was lysed with lysis buffer at pH 7.5 and second with lysis buffer at pH 8.0. The lysates were immunoextracted with mouse anti-V5 antibody as in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057063#s4" target="_blank">Materials and methods</a>”. The immunoprecipitates (Co-IP) and a fraction of total cell lysate (1/10th) were analyzed in Western blots for the presence of STAT1 or STAT2 using rabbit anti-STAT1 and rabbit anti-STAT2 antibodies respectively. The blots from total cell lysates were also probed with mouse anti-V5 antibody for analyzing the expression levels of the expected V proteins. The primary antibodies were detected with peroxidase-labelled anti-mouse or anti-rabbit IgG antibody.