Neutralization by PGT121-137 antibodies and chimeric variants.

<p>Strong neutralization is coded in red (>90%), intermediate in yellow (75–90%) and weak in green (50–75%). Neutralization data were generated in single neutralization experiments, with each test sample analyzed in duplicate to determine the percent decrease in virus growth (neutralization) as compared and normalized to the control wells without antibody expression (defined as 0% neutralization). Each antibody was assayed at a 1∶5 final dilution with virus added. Neutralization results were normalized by the relative antibody expression, with the maximum neutralization defined to be 100%. Note that in cases when the expression level of the antibodies was more than 2-fold different from the average, the normalization is probably not accurate and these numbers have been marked with a star. For comparison, the raw neutralization data has been added as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055701#pone.0055701.s005" target="_blank">Figure S5</a>. Supernatants expressing antibody 17b showed 0% neutralization with HIV-1 TRO.11 and 27% neutralization with HIV-1 SF162.LS; supernatants expressing VRC01 showed 100% neutralization to both HIV-1 viruses (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055701#pone.0055701.s008" target="_blank">Figure S8</a>).