Effect of treatment with selective COX-1 (SC560) or COX-2 (SC236) inhibitors toward PGI₂ production in HUVECs incubated with EGCG.

<p>Cells were incubated in medium 5% FCS in the presence of increasing concentrations of SC560 or SC236 (1–100 nM); 10 min after treatment with inhibitors, cells were added with arachidonic acid (10 µM) and EGCG (100 µM) for 30 min. PGI₂ was evaluated as 6-keto-PGF_1α in aliquots of cell supernatants using a specific EIA. Values are expressed as pg/ml ± SE (<i>n</i> = 3) of 6-keto-PGF_1α. # P<0.01 vs AA alone; *<i>P</i><0.05, **<i>P</i><0.01 <i>vs.</i> (AA+EGCG).