Analysis of the oligomeric state of FIV p24 as a function of the protein concentration.

<p>A) Size distribution profiles as a function of the volume of FIV p24-His at 5 mg/ml (left) and 7 mg/ml (right), by Dynamic Light Scattering. The graph shows the superposition of three successive measurements from 12 runs at each concentration, which are representative for at least three independent experiments. B) Chemical cross-linking of recombinant FIV capsid protein with protein at 5 mg/ml or 7 mg/ml. Lane MW, protein molecular mass marker. A negative control without BS³ has been realized on the same proteins (−BS³). C) Dissociation of FIV p24 dimer as followed by ITC. The thermogram (top panel) and the plotted titration curve (bottom panel) were obtained with a Microcal ITC200. The solid line (bottom panel) represents the fitting of the data by the built-in dimer dissociation model.