IL-33 attenuates anoxia/reoxygenation (A/R)-induced myocyte apoptosis through inhibition of PKCβ.

<p><b>A</b>. Cardiomyocytes were challenged with an A/R with or without IL-33 (1–5 ng/ml) and harvested at the time indicated for PKCβII phosphorylation assay with Western blot. Upper-panel is the actual blots. Lower-panel is the densitometry analysis. n = 3, *p<0.05 compared with N/R without IL-33; ^#p<0.05 compared with respective A/R without IL-33. <b>B</b>. Cardiomyocytes were transfected with siRNA specific to PKCβII, or control siRNA. Subsequently, the myocytes were challenged with an A/R with or without IL-33. The Cardiomycytes were harvested at 6 hrs or 24 hrs after the A/R for caspase 3 or fragmented DNA assay. n = 4, *p<0.05 compared with control siRNA treated myocytes with N/R without IL-33; ^#p<0.05 compared with control siRNA treated myocytes with A/R without IL-33.