Increased motor neuron survival in E15.5 and E18.5 GAD67-deficient (−/−) brachial motor nuclei.

<p>A to D and F to I show light micrographs of the brachial and lumbar motor nuclei from GAD67-deficient (B, D, G, I) and wild type littermate control (A, C, F, H) at E18.5. C-D and H-I show motor neurons within the brachial and lumbar lateral motor columns at higher magnification, respectively. E and J show the mean motor neuron numbers ± SEM of brachial motor neurons (E) and lumbar motor neurons (J) from GAD67-deficient (−/−) and wild type (+/+) littermates respectively, from embryonic day 13.5 (E13.5) through to E18.5. Increased motor neuron survival was observed in GAD67-deficient mice for brachial motor LMC nuclei (E), whereas motor neuron numbers were unchanged for lumbar motor LMC nuclei (J), compared to wild type (<i>n</i> = 6 per age, **<i>P</i><0.01, unpaired <i>t</i> test). Scale Bar: A, B, F, G, 100 µm, C, D, H, I, 50 µm.