Effects of Cholesterol on the Membrane Conductance of CLIC1.

<p>Representative impedance spectroscopy recordings of (A) 7.4 µM CLIC1 (in 100 µL volume) pre-incubated with 2 µL ethanol (solvent control), (B) no protein added (buffer control), (C) 7.4 µM CLIC1 (in 100 µL volume) pre-incubated with 2 µL cholesterol in EtOH, and (D) 14.8 µM CLIC1 (in 100 µL volume) pre-incubated with 2 µL cholesterol in EtOH. Samples added to tethered membranes made from AM199 lipid containing 50 mol% cholesterol; [n = 3].