LMWF protected HK2 cells during hypoxia-reoxygenation.

<p>HK2 cells were exposed to hypoxia for 12 h by using the MGC AnaeroPack System. After hypoxia, cells were maintained in complete medium for 3 h with 1∼20 µg/ml LMWF or MAPK inhibitors, SB-203580 (SB), U-0126 (U), SP-600125 (SP), at 15 µM, or DMSO (D) as a control. (A) Cell viability was assayed using CCK8 kit. Data are mean ± SEM. n = 4. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001. (B) Effect of LMWF and MAPK inhibitors on dissipation of ΔΨm in HK2 cells. Bar diagram shows ratio of red fluorescence to green fluorescence. Data are mean ± SEM. n = 4. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001. (C) Representative blottings (<i>up</i>) and relative ratios of protein levels (<i>down</i>) are shown. Each bar represents the mean ± SEM. n = 3; #<i>P</i><0.05, ##<i>P</i><0.01, ###<i>P</i><0.001 vs. control, *<i>P</i><0.05, **<i>P</i><0.01 vs. hypoxia-reoxygenation group. (D) Representative Western blottings of mitochondrial and cytosolic cyto-c are shown.