Effect of different neurotransmitters on the h-current.

<p>A: effect of NA (100 µM, +1 mM ascorbic acid); the amplitudes at different time points are normalized with respect to the amplitude at time zero; n = 16 (controls) 11 (NA), 6 (clonidine). B: box charts showing the lack of effect of the indicated neurotransmitters;5-HT (50 µM, n = 7),quinpirole (30 µM, n = 14), histamine (10 µM, n = 11), oxotremorine (10 µM, n = 7), baclofen (10 µM, n = 4) and dopamine (100 µM,+1 mM ascorbic acid; n = 8). C: effect of clonidine (α2 agonist, 10 µM) on the resting membrane potential. The membrane was hyperpolarized to −74 mV (by injecting −45 pA) in order to prevent spontaneous firing. All recordings shown in this figure were performed in slice, perforated patch, 37 °C; external saline was EC 3 plus BL 1 and BL 2 mixes for experiments shown in A and B, EC 1 plus BL 1 for the experiment shown in C.