Changes in expression of survival and apoptotic proteins in U87MG and U118MG xenografts.

<p>Mice with xenografts were treated for 11 days. Treatments: control (CTL) did not receive any treatment but tumor bearing mice (10^th day after tumor implantation) were injected with photofrin (10 mg/kg) by tail vein, and 24 h later, 670 nm light was delivered to the tumor with fluencies of 100 J/cm² at a fluency rate of 50 mW/cm². We used 100 J/cm² to expose most of the tumor cells to radiation (32). Then, the mixture of miR-99a mimic (50 µg) and 0.05% atelocollagen in 200 µl was injected (via tail vein) into each mouse on 14^th, 17^th, and 20^th days. All animals were sacrificed on 21^st day. Representative Western blots (<i>n</i>≥3) showed expression of 97 kDa FGFR-3, 185 kDa PI3K, 62 kDa Akt, 53 kDa p53, 35 kDa caspase-9 (active), 20 kDa caspase-3 (active), and 42 kD β-actin.