Screening of discoidin domain receptor 2 (DDR2) siRNA and transfection efficiency of short hairpin RNA plasmid (p.DDR2.shRNA).
- Zheng Luo (284622)
- Huimin Liu (284624)
- Xiaomeng Sun (284626)
- Rong Guo (50063)
- Ruibing Cui (284629)
- Xiangxing Ma (284630)
- Ming Yan (284631)
Publication date
February 2013
DOIAbstract
<p>(a) RT-PCR analysis of mRNA level of DDR2 in siRNA-transfected immortalized rat hepatic stellate cell line HSC-T6. Arrows indicate the position of DDR2 and actin. (b) Quantification of mRNA level of DDR2 relative to that of actin. Data are mean ± SD (n = 3). **P<0.01 <i>vs</i> normal and siRNA control. (c) Light (left) and fluorescence microscopy (right) of transfection efficiency of p.DDR2.shRNA as compared with no plasmid.</p
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