Neuromuscular junctions and expression of nicotinic acetylcholine receptors (nAChRs).

<p>(A) Muscle sections were stained for nAChRs with α-bungarotoxin-FITC (green) and with SV2A antibody (red) to mark pre-synaptic structures. Note the coincident staining of both markers indicating presumptive functional NMJs. DAPI staining (blue) shows nuclei. Scale bar = 25 µm. (B) RT-PCR analysis of the different nAChR subunits (α, β, γ, δ, ε) and muscle specific kinase MuSK in control muscle (con) and muscle from the operated side of animals undergoing immediate (0) or 1,3,6 months delayed nerve repair. 18 S was used as a house-keeping gene.