Labeling of FSK-treated human adipocytes with anti-pPeri-site 5 and anti-pPeri-site 6 antibodies.

<p>Human subcutaneous adipocytes were exposed to either control medium, or medium supplemented with 10 µM FSK and 500 µM IBMX for 2 minutes, then fixed and labeled for nuclei, lipid droplets, and with either anti-pPeri-site 5 or anti-pPeri-site 6. A goat-anti-mouse secondary antibody coupled to Texas Red was used to visualize the phospho-perilipin antibodies in the red fluorescent channel. A, Control adipocytes are shown visualized for nuclei (blue) and lipid droplets (green). B, the same field is shown visualized for anti-pPeri-site 5 (red). C, FSK/IBMX-treated adipocytes are shown visualized for nuclei and lipid droplets. D, The same field is shown visualized for anti-pPeri-site 5. E, Control adipocytes are shown visualized for nuclei and lipid droplets. F, The same field is shown visualized for anti-pPeri-site 6. G, FSK/IBMX-treated adipocytes are shown visualized for nuclei and lipid droplets. H, The same field is shown, visualized for anti-pPeri-site 6. Scale bar = 50 µm.