Effect of LPS and progesterone treatment on uterine nitric oxide levels.

<p>Mice were injected on day 7 of gestation with LPS (1 µg/g) or LPS and progesterone (2 mg/mouse, 2 h before LPS injection). They were sacrificed 6 h after LPS treatment and uterine tissue was cultured for 24 h. Nitric oxide was measured as nitrate (NO₃⁻) plus nitrite (NO₂⁻) accumulation in culture supernatants by Griess technique. n = 5. Values are means ± SEM. Results are expressed as µM NO₂⁻/mg tissue. Bars with different superscript letters denote significant differences (P<0.05). a≠b.