Effect of tariquidar treatment on protein and mRNA expression of MRP7 in HEK/pcDNA and HEK/MRP7 cells.

<p>(A) The effect of 0.3 µM tariquidar treatment on the protein expression of MRP7 in HEK/pcDNA and HEK/MRP7 cells at 0, 4, 12, 24, 48 and 72 h. (B) The effect of 72 h tariquidar treatment at difference concentrations on the protein expression of MRP7 in HEK/pcDNA and HEK/MRP7 cells. (C) mRNA levels of <i>MRP7</i> in HEK/pcDNA and HEK/MRP7 cells treated with 0.3 µM tariquidar at different time points. GAPDH was used as a loading control. The protein or mRNA levels of MDR7 were normalized to those of GAPDH and shown on the right. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055576#s3" target="_blank">Results</a> are represented as mean ± SD. <i>P</i> values were obtained using analysis of variance by comparing the relative amounts of protein or mRNA in cells treated with tariquidar with those in untreated control cells. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055576#s3" target="_blank">Results</a> representative of 3 independent experiments are shown. *: <i>P</i><0.05; **: <i>P</i><0.01.